Single-Molecule Counting on SlipChip Using a Mobile Phone
In the video below, single molecules were amplified on SlipChip and a cell phone was used to image the chip in a shoebox. The image was then sent to a remote server, where the pattern of "positive" and "negative" wells on the chip was automatically analyzed, Poisson statistics was applied, and the number of molecules present in the sample was calculated. Quantitative results were automatically sent via email. A minimally trained user can use this approach.
Reference: David A. Selck, Mikhail A. Karymov, Bing Sun, and Rustem F. Ismagilov, "Increased Robustness of Single-Molecule Counting with Microﬂuidics, Digital Isothermal Ampliﬁcation, and a Mobile Phone versus Real-Time Kinetic Measurements," Analytical Chemistry 2013, DOI: 10.1021/ac4030413.