GOAL: DEVELOP DISRUPTIVE INNOVATIONS FOR GLOBAL HEALTH

Our lab is pioneering the development of advanced diagnostic devices capable of ultrasensitive, quantitative biomarker measurements. Such technologies are poised to revolutionize medicine—providing rapid point-of-care (POC) diagnoses under a variety of settings outside of clinical labs.

One area of active focus in our lab is developing tools capable of rapidly detecting antibiotic susceptibility. The liberal use and misuse of antibiotics has led to an impending global health crisis: the widespread development of antibiotic resistance. Prescribing correctly and achieving antibiotic stewardship both critically depend on health care providers learning the results of an antibiotic susceptibility test (AST) during a patient's visit. Such a test simply does not exist—even in hospital settings. Development of a rapid POC AST was selected for the Longitude Prize as the most urgent science/technology challenge facing humanity, ahead of all other challenges in energy, environment and health. (See also: White House Executive Order, 2015).


[VIDEO LINK] "Diagnostics for Global Health and Antimicrobial Stewardship"
10/26/2016 - PI RUSTEM F. ISMAGILOV (54 min)

 

SOME OF THE CHALLENGES WE WORK TO SOLVE IN OUR LAB


How do we make quantitative, ultrasensitive molecular measurements accessible to anyone, anywhere?

How do we genotype viruses, measure viral load, and detect antibiotic resistance under limited-resource settings?

How do we create devices/architectures that can be operated by untrained users, without a lab or equipment?

What are the mechanisms of single-molecule amplification reactions; how do we design robust amplification schemes?

How can we detect critical biomarkers at unknown (very high to very low) concentrations?

 

SOME OF OUR RECENT PUBLICATIONS


 

Schoepp et al. 2016. Digital Quantification of DNA Replication and Chromosome Segregation Enables Determination of Antimicrobial Susceptibility After Only 15 Minutes of Antibiotic Exposure. Angew Chemie. doi:10.1002/anie.201602763.

Rodriguez-Manzano et al. 2016. Reading Out Single-Molecule Digital RNA and DNA Isothermal Amplification in Nanoliter Volumes with Unmodified Camera Phones. ACS NANO. 10(3): 3102-3113.

Jue et al. 2016. Evaluating 3D printing to solve the sample-todevice interface for LRS and POC diagnostics: example of an interlock meter-mix device for metering and lysing clinical urine samples. Lab on a Chip 16: 1852-1860. VIDEO LINK

Khorosheva et al. 2015. Lack of correlation between reaction speed and analytical sensitivity in isothermal amplification reveals the value of digital methods for optimization: validation using digital real-time RT-LAMP. NAR.

Ge et al. 2014. Digital, Ultrasensitive, End-Point Protein Measurements with Large Dynamic Range via Brownian Trapping with Drift. JACS 136:14662-14665.

Begolo et al. 2014. The Pumping Lid: investigating multi-material 3D Printing for Equipment-Free, Programmable Generation of Positive and Negative Pressures for Microfluidic Applications. Lab Chip. 14(24):4616-4628.

 

 


We are currently hiring postdoctoral researchers!

Please see our "Positions" page for more details.